The conference chairs, the organizing committee and the Engineering Foundation would like to thank the following organizations for their financial support of this conference.

Saturday, February 7, 1998

3:00 p.m. - 9:00 p.m. Registration and Check-In (Lobby)

5:30 p.m. - 7:00 p.m. Dinner

7:00 p.m. - 10:00 p.m. Conference Opening and Welcome
Dr. Robert Arathoon, Conference Co-Chair
Professor Jeff Chalmers, Conference Co-Chair
Allen Laskin, Engineering Foundation

7:15 p.m. - 7:45 p.m. HISTORICAL PERSPECTIVE
"Historic Perspective in Cell Culture Engineering"
Professor Daniel I. C. Wang
Massachusetts Institute of Technology

7:45 p.m. - 8:15 p.m. Dr. Tony Lubiniecki
SmithKline Beecham

8:15 p.m.- 10:00 p.m. Opening Reception/Social Hour

Sunday, February 8, 1998

7:00 a.m. - 8:30 a.m. Breakfast

8:30 a.m. QUANTIFICATION APPROACHES IN LARGE SCALE CELL CULTURE
Session Co-Chairs:
Professor Carol Heath, Iowa State University
Dr. Weichang Zhou, Merck Research Industries

8:35 a.m. - 9:05 a.m. "From Applied Research to Industrial Application:
The Monitoring of Intracellular Necleotides as a Useful Tool in the Development and Characterization of Mammalian Cell Culture Processes"
Dr. Stephanos T. Grammatikos
Dr. Karl Thomae GMBH

9:05 a.m. - 9:35 a.m. "Comparison of Large Scale Batch and Continuous Perfusion Processes for Production of Mammalian Cell Derived Recombinant Proteins"
Gregory MacMichael
Medarex & Chiron Corp.

9:35 a.m. - 10:05 a.m. "Characterization of Cellular Nutrition Requirements, Cell Metabolism and Cell Death Mechanism of a Fed-Batch Recombinant NSO Culture"
Weichang Zhou
Merck Research Laboratories

10:05 a.m. - 10:35 a.m. "Improved Glycosylation of Recombinant Proteins Produced in CHO Cells Overexpressing Human
b1,4-galactosyltransferase and/or a 2,3-sialtransferase"
Dr. Lynne Krumme
Genentech, Inc.

10:35 a.m. - 11:00 a.m. Coffee Break

11:00 a.m. - 11:30 a.m. "Multiple Steady States in Continuous Cultivation of Hybridoma Cells"
Professor Wei-Shou Hu
University of Minnesota

11:30 a.m. - 12:00 noon "Regulation of NCAM Polysialylation by pCO2 and pH in Culture"
Professor William Miller
Northwestern University

12:00 noon - 12:30 p.m. "The "Push-to-Low" Approach for Feeding Optimization of High-Density Perfusion Animal Cell Culture"
Dr. Konstantin Konstantinov
Bayer Corp.

12:30 p.m. - 2:00 p.m. Lunch

2:00 p.m. - 4:00 p.m. Ad hoc Session/Free Time

4:00 p.m. - 5:00 p.m. POSTER SESSION I

5:00 p.m. - 5:30 p.m. Special Lecture for Cell Identification, Selection and Separation
"Antigen Characterization of Breast Cancer Cells"
Dr. M. Mitchell
University of California at San Diego

5:30 p.m. - 7:30 p.m. PRODUCT QUANTIFICATION AND FACTORS IN CELL CULTURE THAT AFFECT PRODUCT QUALITY
Session Co-Chair:
Dr. Kenneth Seamon, Immunex Corporation
Dr. Sadettin Ozturk, Bayer Corp.

5:30 p.m. - 6:00 p.m. "Regulatory Issues in Evaluating Product Quality in Cell Culture"
Dr. J. O'Connor
Genentech Inc.

6:00 p.m. - 6:30 p.m. "Product Quality Characteristics and Impact of Cell Culture Parameters"
James N. Thomas
Immunex Corporation

6:30 p.m. - 7:00 p.m. "Characterization of the Expression Construct in Cells Used for Production Benefix"
Dr. Leonard
Genetics Institute

7:00 p.m. - 7:30 p.m. "Ammonia Affects the N-Glycan Structures of Recombinant Proteins: Exploring the Mechnisms"
Dr. Martin Gawilitzek
Genentech Inc.

7:30 p.m. - 9:00 p.m. Dinner

9:00 p.m. - 10:00 p.m. Social Hour

Monday, February 9, 1998

7:00 a.m. - 8:30 a.m. Breakfast

8:30 a.m. - 12:00 Noon USE OF ANIMAL DERIVED COMPONENTS IN CELL CULTURE
Session Chair:
Dr. Tony Lubiniecki, SmithKline Beecham

8:30 a.m. - 9:00 a.m. "An Overview of Viral and Virus-Like Agents in Cell Culture Systems"
Dr. John Petricciani
International Association for Biological Standardization

9:00 a.m. - 10:00 a.m. KEYNOTE LECTURE
"Prion Biology and Diseases - Potential Problems in the Preparation of Prion Free Therapeutics"
Professor Stan Prusiner
University of California, San Francisco

10:00 a.m. - 10:30 a.m. Coffee Break

10:30 a.m. - 11:00 a.m. "PhRMA Risk Assessment Study for BSE in Animal-Derived Raw Materials"
Dr. Frederic G. Bader
Centocor, Inc.

11:00 a.m. - 11:30 a.m. "Collective Experiences of Adventitious Viruses of Animal
Derived Raw Materials and What Can Be Done About Them"
Dr. R. Levings
USDA APHIS, Ames, Iowa

11:30 a.m. - 12:00 noon "Approaches to Prevention of Virus Contamination of Cell Culture Medium"
Dr. Gian Polastri
Genentech, Inc.

12:00 noon - 1:30 p.m. Lunch

1:30 p.m. - 3:00 p.m. Ad hoc Session/Free Time

3:00 p.m. - 5:00 p.m. WORKSHOP:
MONITORING AND CONTROL OF CELL CULTURE PROCESSES
Lawrence Forman, Forman Consulting
Georg Schmid, F. Hoffman LaRoche

5:00 p.m. STEM CELLS: DIFFERENT SYSTEMS AND ENGINEERING
Session Co-Chairs:
Professor William Miller, Northwestern University
Professor David Humes, University of Michigan

Introduction and Overview
Professor William Miller

5:00 p.m. - 5:30 p.m. "Use of Kidney Stem Cells to Engineer a Bioartificial Tubule"
Professor David Humes
University of Michigan

5:30 p.m. - 6:00 p.m. "Building Blood Vessels de Novo"
Professor Charles Little
Medical University of South Carolina

6:00 p.m. - 6:30 p.m. "Neural Stem Cells"
Professor John Kessler
Albert Einstein College of Medicine

6:30 p.m. - 7:00 p.m. "Modeling Organogenesis Using Chemical Engineering Methaphors"
Professor Lars Nielsen
University of Queensland

7:00 p.m. - 7:15 p.m. Concluding Remarks
Professor David Humes
University of Michigan

7:30 p.m. - 9:00 p.m. Dinner

9:00 p.m. - 10:00 p.m. Social Hour

Tuesday, February 10, 1998

7:00 a.m. - 8:30 a.m. Breakfast

8:30 a.m. - 12:00 Noon CELL ENGINEERING FOR CELL CULTURE
Session Co-Chairs
Professor Eleftherios T. Papoutsakis, Northwestern University
Professor Peter Gray, University of New South Wales

8:30 a.m. - 9:15 a.m. "Metabolic Engineering of the CHO Cell Cycle by Regulated Expression of Critical Genes"
Professor Jay Bailey
ETH, Zurich

9:15 a.m. - 9:45 a.m. "The Effect of Recombinant Insulin-Like Growth Factor One (IGF-1) Production on the Expression of IGF-1 Receptors and the Interaction With IGF-1 Binding Proteins in the Super- CHO Cell Line"
Professor Peter Gray
University of New South Wales

9:45 a.m. - 10:15 a.m. Coffee Break

10:15 a.m. - 10:45 a.m. "Engineering CHO Cells to Achieve a Non-Growth-Associated Production of Foreign Proteins"
Professor Dhinakar Kompala
University of Colorado

10:45 a.m. - 11:15 a.m. "Suppressing The Cell Death Cascade in Virally Infected Mammalian Cells"
Professor Michael Betenbaugh
Johns Hopkins University

11:15 a.m. - 11:45 a.m. "Restructuring the Carbohydrates of Recombinant Glycoproteins"
Professor Nigel Jenkins
DeMontfort University

12:00 noon - 1:30 p.m. Lunch

1:30 p.m. - 3:00 p.m. POSTER SESSION I

3:00 p.m. - 5:30 p.m. Activities at Balboa Park

5:30 p.m. - 7:30 p.m. Dinner at Balboa Park

7:30 p.m. Return to Hotel

8:00 p.m. - 9:00 p.m. KEYNOTE ADDRESS
"Of Genes and Genomes"
Professor David Botstein
Stanford University

9:00 p.m. - 10:30 p.m. POSTER SESSION II with Social Hour

Wednesday, February 11, 1998

7:00 a.m. - 8:30 a.m. Breakfast

8:30 a.m. - 12:00 Noon CELL IDENTIFICATION, SELECTION AND SEPARATION
Session Co-Chairs
Professor Eleftherios T. Papoutsakis, Northwestern University
Dr. Jim Bender, Baxter Healthcare

8:30 a.m. - 9:10 a.m. "Identification of Therapeutic Cell Populations Using Monoclonal Antibodies Directed at Cell Surface Antigens"
Dr. Jim Bender
Baxter Healthcare

9:10 a.m. - 9:50 a.m. "Fast Cell Separation With Immunomagnetic Colloid and Flow-Through Magnetic Sorter"
Dr. Maciej Zborowski
The Cleveland Clinic Foundation

9:50 a.m. - 10:20 a.m. Coffee Break

10:20 a.m. - 10:50 a.m. "Development of An Analytical Magnetophoresis System for the Quantitation of the Magnetic Susceptibility of Immunomagnetically Labeled Hematopoietic Stem Cells"
Dr. Richard Schwartz
SyStemix Inc.

10:50 a.m. - 11:20 a.m. "Mammalian Cell Separation by Ultrasonic Forces"
Dr. James M. Piret
University of British Columbia

11:30 a.m. - 1:00 p.m. Lunch

1:30 p.m. - 3:00 p.m. Ad hoc Session/Free Time

3:00 p.m. - 5:00 p.m. POSTER SESSION II

5:00 p.m. - 7:30 p.m. STRESS IN BIOREACTORS
Session Co-Chairs:
Professor James Piret, University of British Columbia
Dr. Konstantin Konstantinov, Bayer Corp.

5:00 p.m. - 5:40 p.m. "Differential Gene Regulation Induced by Flow in Vascular Cells: Implications for Gene Therapy"
Professor Larry V. McIntire
Rice University

5:40 p.m. - 6:05 p.m. "The Interplay of Agitation Intensity and Media Additives on Receptor Expression"
Professor Eleftherios T. Papoutsakis
Northwestern University

6:05 p.m. - 6:30 p.m. "Variable Functions of bcl-2 in Mediation Bioreactor Stress and Nutrient Deprivation-Induced Apoptosis in Hybridoma Cells"
Mohamed Al-Rubeai
University of Birmingham

6:30 p.m. - 6:55 p.m. "A Framework for the Study of Cell Death in Culture"
Professor Gregory Stephanopoulos
Massachusetts Institute of Technology

6:55 p.m. - 7:20 p.m. "Fatty Acids Enhance the Robustness of Mammalian Cells in Agitated Cultures"
Professor Michael Butler
University of Manitoba

7:30 p.m. - 9:00 p.m. Dinner

9:00 p.m. - 10:00 p.m. Social Hour

Thursday, February 12, 1998

7:00 a.m. - 8:30 a.m. Breakfast

8:30 a.m. - 10:20 a.m. ANTIBODY ENGINEERING
Session Co-Chairs:
Dr. Paul Carter, Genentech, Inc.
Dr. Robert Arathoon, Genentech, Inc.

8:30 a.m. - 8:55 a.m. "TBA"
Kathryn Stein
FDA

8:55 a.m. - 9:20 a.m. "XenoMouseTM - A Robust Technology for Production of Therapeutic Human Monoclonal Antibodies"
Dr. Aya Jakobovits
Abgenix, Inc.

9:20 a.m. - 9:55 a.m. "Human Antibodies from Phage"
Dr. P.R. Tempest
CAT, UK

9:55 a.m. - 10:20 a.m. "Clinical Development of RituxanTM (IDEC-C2B8): First Monoclonal Antibody for the Treatment of Lymphoma"
Dr. Antonio J. Grillo-Lopez
IDEC Pharmaceuticals Corporation

10:20 a.m. - 10:45 a.m. Coffee Break

10:45 a.m. - 12:25 p.m. VIRAL VECTORS AND RAPID EXPRESSION SYSTEMS
Session Co-Chairs
Dr. John Aunins, Merck & Co.
Dr. Amy Murmane, SmithKline Beecham Pharma

10:45 a.m. - 11:10 a.m. "Production Issues for Adenoviral Gene Therapy Vectors"
Dr. Kathleen Hehir
Genzyme Inc.

11:10 a.m. - 11:35 a.m. "Applications of GFP (Green Fluorescent Protein) to Monitor Transient Gene Expression in Cell Culture Processes"
Dr. Amy Murnane
SmithKline Beecham Pharma

11:35 a.m. - 12:00 noon "Transient Transfection in Mammalian Cells A Basic Study for an Efficient and Cost-Effective Scale Up Process"
Dr. Ernst-Jurgen Schlaeger
F. Hoffmann La Roche Ltd.

12:00 noon - 12:25 p.m. "Evolution of Adenovirus Vectors"
Professor R. Parks
McMaster University

12:15 p.m. Lunch and Adjournment

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