Conference Chair
Jens Nielsen
Technical University of Denmark
Conference Co-Chairs
James Liao
University of California Los Angeles
Kristala Jones Prather
Merck Research Labs
Sheri Nidositko
Merck Research Labs
UNITED ENGINEERING FOUNDATION, INC.
Three Park Avenue, 27th Floor
New York, NY 10016-5902
T: 1-212-591-7836 - F: 1-212-591-7441
E: engfnd@aol.com - www.engfnd.org/2ay.html
PROGRAM COMMITTEE
Dr. Roel Bovenberg, DSM, The Netherlands
Dr. Brian Cali, Microbia, USA
Dr. Douglas Cameron, Cargill, USA
Professor Stephen Drew, Princeton University, USA
Dr. Suan Choo Cheah, MPOB, Malaysia
Professor Jean Marie Engasser, Polytechnique de Lorraine, France
Professor David Fell, Oxford Brooks University, UK
Dr. Michael Fonstein, Integrated Genomics, USA
Professor Daryl Granner, Vanderbilt University, USA
Dr. Stéphane Guillouet, INSA Toulouse, France
Professor Joseph J. Heijnen, Delft University of Technology
Professor Wei Shou Hu, University of Minnesota, USA
Dr. Gjalt Huisman, Codexis, USA
Professor Jay Keasling, University of California-Berkeley, USA
Dr. Sang Yup Lee, KAIST, South Korea
Dr. Philip Lessard, Massachusetts Institute of Technology, USA
Dr. Eric Mathur, Diversa, USA
Dr. Vasantha Nagarajan, Dupont, USA
Professor Bernhard Palsson, University of California San Diego, USA
Professor Hermann Sahm, Institute of Biotechnology-Jülich, Germany
Dr. Karl Sanford, Genencor, USA
Dr. Frank Skraly, Metabolix, USA
Dr. Chuck Squires, Dow Chemical Company, USA
Professor John Villadsen, Technical University of Denmark
Professor Hans Westerhoff, Free University of Amsterdam, Netherlands
Dr. Oskar Zelder, BASF, Germany
STEERING COMMITTEE
The following members provide continuing direction in the development and
focus of the Metabolic Engineering Conferences in association with the
United Engineering Foundation:
Professor Anthony J. Sinskey, Massachusetts Institute of Technology,
USA
Professor Matthias Reuss, University of Stuttgart, Germany
Professor Gregory Stephanopoulos, Massachusetts Institute of Technology,
USA
Dr. Barry Buckland, Merck Research Laboratories, USA
Sunday, 6 October
14:00 Registration
18:00 - 19:00 Opening Session
Welcome: Jens Nielsen, Conference Chair
Allen Laskin, UEF
18:15 Keynote Lecture
The role of metabolic engineering in the chemical industry - can MPE deliver?
Karl Sanford, Genencor, USA
19:00 - 19:30 Welcome Reception
19:30 - 21:00 Dinner
21:00 - 22:00 Social Hour
Monday, 7 October
07:30 - 08:30 Breakfast
08:30 - 12:30 Session 1: Metabolites from microorganisms
Chairs: Karl Sanford, Genencor, USA
Uwe Sauer, ETH-Zürich, Switzerland
08:30 Gene expression response to isoleucine overproduction in Corynebacterium
Phil Lessard, MIT, USA
09:00 What makes a good 2-keto L gulonate producer?
Fernando Valle, Genencor, USA
09:30 Metabolic engineering of riboflavin production in Bacillus subtilis
Peter Hohmann, Roche Vitamins, Switzerland
10:00 Eau-de-E. coli: Production of flavor and fragrance terpenes
in Escherichia coli
Vincent Martin, UC Berkeley, USA
10:30 Coffee Break
11:00 Metabolic engineering of central metabolism in S. cerevisiae
Jack Pronk, TUD, The Netherlands
11:30 Metabolic pathway engineering for the production of fine chemicals
Oskar Zelder, BASF AG, Germany
12:00 Metabolic flux analysis and metabolic engineering for improved lysine
production
Daniela Kruse, Degussa, Germany
12:30 - 14:00 Lunch
14:00 - 15:00 Free time
15:00 - 18:30 Session 2: High throughput analysis in metabolic systems
Chairs: Tony Sinskey, MIT, USA
Jack Pronk, TUD, The Netherlands
15:00 Impact of genomics on metabolic engineering
James Liao, UCLA, USA
15:30 High throughput analysis of lactic acid bacteria
Willem de Vos, Wageningen University, The Netherlands
16:00 Integrated analysis of E. coli and its potential for directing
metabolic engineering
Robert LaRossa, DuPont, USA
16:30 Coffee Break
17:00 Proteome and transcriptome analysis of Bacillus subtilis
Jörg Stülke, University of Erlangen, Germany
17:30 High-throughput analysis of stem-cell differentiation and
T-cell expansion
Terry Papoutsakis, Northwestern University, USA
18:00 Use of 13C-labelled substrates for rapid phenotypic characterization
of recombinant strains
Bjarke Christensen, Novozymes, Denmark
19:30 Dinner
21:00 - 23:00 Poster Session I / Social Hour
Chairs: Stephen Van Dien, Ajinomoto, Japan
George Ruijter, Leiden University Medical Center, The Netherlands
Tuesday, 8 October
07:30 - 08:30 Breakfast
08:30 - 12:00 Session 3: Metabolic engineering of prokaryotes
Chairs: Vasantha Nagarajan, Dupont, USA
Oskar Zelder, BASF, Germany
08:30 Expression of complex proteins in bacteria: Genetic, structural
biology and engineering analysis of a novel pathway
George Georgiou, University of Texas, USA
09:00 Metabolic engineering of maintenance metabolism improves metabolite
production in Bacillus subtilis
Uwe Sauer, ETH Zürich, Switzerland
09:30 Metabolic engineering of glycopeptide antibiotics
Stefano Donadio, Biosearch Italia, Italy
10:00 Coffee Break
10:30 Metabolic engineering of E. coli to produce D-phenylglycine
(D-PG) or D-p-hydroxyphenylglycine (D-HPG) by fermentation
Ulrike Müller, DSM Biotech GmbH, Germany
11:00 Hyaluronic acid production by Streptococci
Lars K. Nielsen, University of Queensland, Australia
11:30 Production of 1,3 propanediol by E. coli
Charles Nakamura, Dupont, USA
12:30 - 13:30 Lunch
13:30 - 16:30 Session 4: Biocatalysis
Chairs: Douglas Clark, University of California, Berkeley, USA
Jonathan Dordick, Rensselaer Polytechnic Institute, USA
13:30 Laboratory evolution of carotenoid biosynthetic pathways
Daisuke Umeno,
Cal Tech, USA
14:00 In vitro reconstruction of biochemical pathways on
the micro-scale and nano-scales
Jonathan S. Dordick, Rensselaer Polytechnic Institute, USA
14:30 Biocatalysis for the production of chiral compounds in the pharmaceutical
industry
Sheri Nidositko, Merck Research Laboratories, USA
15:00 Coffee Break
15:30 Biocatalytic synthesis of naturally inspired libraries
Douglas Clark, University of California Berkeley, USA
16:00 Biocatalysts for novel biopolymers
Alexander Steinbüchel, Münster University, Germany
16:30 Break
17:00 - 19:00 Parallel Workshops
17:00 Quantitative experimental analysis: The challenge of multiplexing
Chairs: Stéphane Guillouet, INSA, France
Maria Klappa, University of Maryland, College Park, USA
17:00 Computational methods: The challenge of integration
Chairs: Mats Åkesson, DTU, Denmark
Walter van Gulik, TUD, Netherlands
19:30 Dinner
21:00 - 23:00 Poster Session I (continued) / Social Hour
Wednesday, 9 October
09:00 - 12:10 Session 5: Metabolic engineering of quality traits in plants
Chairs: Phil Lessard, MIT, USA
Jackie Shanks, Iowa State University, USA
09:00 Metabolic engineering of flux into methionine and S-adenosylmethione
using hybrid yeast-plant MTHFR
Sanja Roje, University of Florida, USA
09:40 Metabolic engineering of natural products in Arabidopsis thaliana - implications
for gene regulation, metabolic alterations and plant defense
Søren Bak, Royal Veterinary and Agricultural University, Denmark
10:20 Coffee Break
10:50 Pathway characterization in potato tubers using metabolite profiling
Alisdair
Fernie, Max-Planck-Institute fur Molekulare Pflanzenphysiologie, Germany
11:30 Metabolic engineering for improved quality traits in soybean seeds
Eve Syrkin Wurtele, Iowa State University, USA
12:30 Buses leave for conference excursion to Lucca
Lunch bags provided
19:30 Dinner
21:00 - 23:00 Poster Session II / Social Hour
Chairs: Mhairi McIntyre, DTU, Denmark
Jeremy Edwards, University of Delaware, USA
Thursday, 10 October
07:30 - 08:30 Breakfast
09:00 - 12:00 Session 6: Inverse metabolic engineering
Chairs: Brian Cali, Microbia, USA
Lisbeth Olsson, DTU, Denmark
09:00 Use of functional genomics in reverse metabolic engineering
Kevin Madden, Microbia, USA
09:30 Genome breeding of amino acid producing Corynebacterium glutamicum
M. Ikeda, Tokyo Research Laboratories, Japan
10:00 Metabolic engineering for increased galactose uptake in S. cerevisiae
Lisbeth Olsson, DTU, Denmark
10:30 Coffee Break
11:00 A post-genomics strategy for inverse metabolic engineering of microbial
fitness
Ryan Gill, University of Colorado, USA
11:30 Characterization of the phosphate starvation response in Corynebacterium
glutamicum: DNA microarray analyses reveal the corresponding stimulon and
regulon
Volker F. Wendisch, Forschungszentrum Jülich, Germany
12:30 - 13:30 Lunch
13:30 - 15:00 Free time
15:00 - 18:00 Session 7: Systems analysis in metabolic engineering
Chairs: Gregory Stephanopoulos, MIT, USA
Michael Fonstein, Integrated Genomics, USA
15:00 Bringing genomes to life: The use of in silico models
Bernhard Palsson, UCSD, USA
15:30 In silico pathway analysis and discovery
Costas Maranas, Pennsylvania State University, USA
16:00 Application of metabolome data for model reconstruction
Matthias Reuss, University of Stuttgart, Germany
16:30 Coffee Break
17:00 Systems analysis and design of metabolic regulatory circuits
Vassily Hatzimanikatis, Northwestern University, USA
17:30 Comparative genomics of pathogenic bacteria
Michael Fonstein, Integrated Genomics, USA
18:00 - 18:30 Break
18:30 - 19:00 Pre-Banquet Reception
19:00 - 20:00 Merck Award in Metabolic Engineering
Chair: Barry Buckland, Merck Research Laboratory, USA
Metabolic engineering in a rapidly changing world
Gregory Stephanopoulos, MIT, USA
20:00 Conference Banquet
Awards Ceremony
Jay Bailey Young Investigators Award
Merck Poster Awards
UEF Fellowships
22:00 - 23:00 Poster Session II (continued) / Social Hour
Friday, 11 October
07:30 - 08:30 Breakfast
08:30 - 12:30 Session 8: Metabolic engineering of cell cultures
Chairs: Wei-Shou Hu, University of Minnesota, USA
Konstantin Konstaninov, Bayer Corporation, USA
08:30 Strategies for limiting apoptosis in mammalian cell cultures
Mike Betenbaugh, Johns Hopkins University, USA
09:00 Heterologous gene control systems and their use for molecular interventions
in mammalian cells
Martin Fussenegger, ETH-Z, Switzerland
09:30 Genomic exploration in cell culture engineering
Wei-Shou Hu, University of Minnesota, USA
10:00 Towards industrial application of real-time metabolic flux analysis for
animal cell culture
Chetan Goudar, Bayer Corporation, USA
10:30 Coffee Break
11:00 Keynote Lecture
Chair: John Villadsen, DTU, Denmark
11:00 Metabolic engineering and human physiology: Our links to small molecules
Joanne K. Kelleher, George Washington University, USA
11:45 Understanding PHA processes in vivo for metabolic engineering
applications
Tony Sinskey, MIT, USA
12:30 - 14:00 Lunch
Conference Close and Departure on buses to Pisa
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