Sunday, October 22, 2000

15:00 - 18:30 Registration

18:30 - 19:00 Welcome and Conference Overview
Tony Sinskey, Conference Chair
Barry Buckland, Conference Co-chair
Allen Laskin, UEF

19:00 - 20:00 OPENING ADDRESS:
Willem Stemmer
Vice-President R&D, Maxygen, Inc., USA
Engineering of Complex Systems by Molecular Breeding

20:00 - 21:30 Dinner

21:30 - 22:30 Social Hour

Monday, October 23, 2000

06:30 - 08:30 Buffet Breakfast

08:30 - 12:00 SESSION I
EXPERIMENTAL AND COMPUTATIONAL TOOLS
Session Chairs
Jean-Marc Engasser, Institute National Polytechnique, FRANCE
David Fell, Oxford Brookes University, UNITED KINGDOM

08:30 - 08:35 Session Chair Introduction: Jean-Marc Engasser

08:35 - 09:05 Metabolic Rate Modeling of Industrial Fermentations and Novel
Bioprocesses
David Mousdale
Bioflux, SCOTLAND

09:05 - 09:35 A Universal Software Framework for Metabolic Flux Analysis
Wolfgang Wiechert
University of Siegen, GERMANY

09:35 - 10:05 Metabolome Analysis: A Powerful Tool in Metabolic Engineering and
Functional Analysis
Jens Nielsen
Technical University of Denmark, DENMARK

10:05 - 10:35 Coffee Break

10:35 - 10:40 Session Chair Introduction: David Fell

10:40 - 11:10 From Analysis to Synthesis: Metabolic Engineering of Saccharomyces cerevisiae
Matthias Reuss
University of Stuttgart, GERMANY

11:10 - 11:40 Unifying Kinetic and MCA Based Models in Metabolic Engineering
J.J. Heijnen
Delft University of Technology, THE NETHERLANDS

11:40 - 12:00 Discussion

12:00 - 13:30 Lunch

13:30 - 16:00 Ad hoc discussions/free time
Poster Session I set up

16:00 - 16:30 Afternoon Coffee/Refreshments

16:30 - 20:00 SESSION II
EVOLUTIONARY STRATEGIES AND METABOLIC ENGINEERING
Session Chairs
Charles Squires, Dow Chemical Corp., USA
Gjalt Huisman, Maxygen, USA

16:30 - 17:00 Understanding How Nature Re-Engineers Proteins for New Functions
Patricia Babbitt
University of California-San Francisco, USA

17:00 - 17:30 Stochastic Modeling and Optimization of Directed Evolution Protocols
Costas D. Maranas
The Pennsylvania State University, USA

17:30 - 18:00 Purification, cDNA Cloning, and Improvement of Activity by Directed
Evolution of a Novel Ketoreductase from Zygosaccharomyces rouxii
Charles Hershberger
Eli Lilly & Company, USA

18:00 - 18:30 Directed Evolution of Sulfur Heterocycle Metabolism Through Flux
Analysis, "Classical" Genetic Engineering, Gene Shuffling and Lucky Guesses
Phil DiGrazia
Enchira Biotechnology Corp., USA 18:30 - 19:00 Directed Molecular Evolution for Metabolic Engineering
Gjalt Huisman
Maxygen, USA

19:00 - 19:30 Evolutionary Engineering of Multiple-Stress Resistant Saccharomyces
cerevisiae
Uwe Sauer
ETH Zürich, SWITZERLAND

19:30 - 20:00 Discussion

20:00 - 21:30 Dinner

21:30 - 22:30 Poster Session I / Social Hour
Session Co-Chairs
Sheri Nidositko, Merck Research Labs., USA
Frank Chaplen, Oregon State University, USA

Tuesday, October 24, 2000

06:30 - 08:30 Buffet Breakfast

08:30 - 12:00 SESSION III
BIOINFORMATICS AND METABOLIC ENGINEERING
Session Chairs
Gregory Stephanopoulos, Massachusetts Institute of Technology, USA
Hans Westerhoff, Free University, THE NETHERLANDS

08:30 - 09:00 Filling in the Missing Pieces of of Biochemistry: The Role of Bioinformatics
Evgeni Selkov
Integrated Genomics, USA

09:00 - 09:30 Metabolic Pathway Databases and Software Tools
Pedro Romero
SRI International, USA

09:30 - 10:00 Cellular Bioinformatics: From Transcriptome to Metabolome … and
Back
Hans V. Westerhoff
Free University, NETHERLANDS

10:00 - 10:30 Coffee Break

10:30 - 11:00 Sequence-Structure-Function Mapping in Microbial Genomes
Shankar Subramanian
University of California at San Diego, USA

11:00 - 11:30 From Genes to Dynamic Molecular Networks
Roland Somogyi
Molecular Mining Corp., CANADA

11:30 - 12:00 Discussion

12:00 - 13:30 Lunch

13:30 - 16:00 Poster Session I (continued)

14:00 - 16:00 Workshop I
Applications of Microarray Technologies to ME
Chair: Philip Lessard, Massachusetts Institute of Technology

16:00 - 16:30 Afternoon Coffee/Refreshments

16:30 - 19:45
SESSION IV
EXPRESSION, COMPLEXITY, AND METABOLIC ENGINEERING
Session Chairs
Douglas Cameron, Cargill, USA
John Villadsen, Technical University of Denmark, DENMARK

16:30 - 17:00 Metabolic Control Engineering - A Post-Genomic Challenge
James Liao
University of California-Los Angeles, USA

17:00 - 17:30 Global Expression Analyses in C. glutamicum and E. coli
Steffen Schaffer
Institut biotechnologie, Jülich, GERMANY

17:30 - 18:00 Transcription Profiling for the Physiological Characterization of
Industrial Microorganisms
Bettina Kempf
BASF Lynx Bioscience AG, GERMANY

18:00 - 18:15 Stretch and Break

18:15 - 18:45 Metabolic Engineering with Artificial Transcription Factors
Casey Case
Sangamo Biosciences, USA

18:45 - 19:15 Expressional Analysis of Escherichia coli Genome During Low and High
Cell Density Growth
Yea-Tyng Yang
Rice University, USA

19:15 - 19:30 Discussion

19:30 - 21:00 Dinner

21:00 - 22:30 Social Hour/Last chance to see Posters from Session I

Wednesday, October 25, 2000

06:30 - 08:30 Buffet Breakfast

08:30 - 12:00 SESSION V
APPLICATION OF METABOLIC ENGINEERING TO PHARMACEUTICALS AND FINE CHEMICALS
Session Chair
Herman Sahm, Institut Biotechnologie, GERMANY

08:30 - 09:20 Directing the Stereoselective Synthesis of Indandiol in Rhodococcus
Philip Lessard and Daniel Stafford
Massachusetts Institute of Technology, USA

09:20 - 10:00 Engineered Microbes as Catalysts in Asymmetric Synthesis
Jon Stewart
University of Florida, USA

10:00 - 10:30 Coffee Break

10:30 - 11:10 Metabolic Engineering of â-Lactam Antibiotic Producing Fungi
Roel Bovenberg
DSM-Group. NETHERLANDS

11:10 - 11:50 A Novel Ca-D-pantothenate Preparation Manufactured by Large Scale
Fermentation
Walter Pfefferle
Dagussa-Hüls AG, GERMANY

11:50 - 12:00 Discussion

12:00 - 13:30 Lunch

12:00 - 14:00 Editorial Board meeting of Journal of Metabolic Engineering (by invitation)

13:30 - 16:00 Ad hoc discussions/free time
Poster Session II set up

14:00 - 15:00 Planning meeting for Metabolic Engineering IV

14:00 - 16:00 Workshop II
Case Studies in Application of ME to Pharmaceuticals and Fine Chemicals
Chairs
Uwe Sauer, ETH Zürich
Peter Salmon, Merck & Co, Inc.

16:00 Afternoon Coffee/Refreshments

16:30 - 19:00
SESSION VI
METABOLIC ENGINEERING: APPLICATIONS TO MEDICINE
Session Chairs
Martin Yarmush, Massachusetts General Hospital, Harvard Medical School, USA
Daryl Granner, Vanderbilt University. USA

16:30 - 17:00 Improving Suicide Gene Therapy by Enzyme and Pathway Engineering
Margaret E. Black
Washington State University, USA

17:00 - 17:30 Metabolic Flux Analysis of Hepatic Metabolic Response to Injury in the
Perfused Liver
Martin Yarmush
Harvard Medical School, USA

17:30 - 18:00 Metabolic Control Analysis and Mitochondrial Pathologies
Jean-Pierre Mazat
Bordeaux University, FRANCE

18:00 - 18:30 New Insights into Epidermal Growth Factor (EGF) Signal Transduction
via Mathematical Modeling
Birgit Schoeberl
Max-Planck Institute, GERMANY

18:30 - 19:00 Discussion

20:00 - 21:30 Dinner

21:30 - 23:00 Poster Session II / Social Hour
Session Co-Chairs
Kristala Jones, Merck Research Labs., USA
Jay Keasling, University of California, USA

Thursday, October 26, 2000

06:30 - 08:30 Buffet Breakfast

08:30 - 12:00 SESSION VII
METABOLIC ENGINEERING OF PLANTS
Session Chairs
Raviga Sambanthamurthi, MPOB, MALAYSIA
John Shanklin, Brookhaven National Laboratory, USA

08:30 - 09:00 Metabolic Engineering of Plants for Osmotic Stress Resistance
Andrew Hanson
University of Florida, USA

09:00 - 09:30 Challenges in Engineering Overproduction of Plant Alkaloids
Jacqueline V. Shanks
Iowa State University, USA

09:30 - 10:00 Genetic Engineering of Primary Carbohydrate Metabolism Within the
Potato (Solanum tuberosum)
Alisdair R. Fernie
Max-Planck Institute, GERMANY

10:00 - 10:30 Coffee Break

10:30 - 11:00 Controlling Modification of Fatty Acids for Improved Plant Oils
John Shanklin
Brookhaven National Laboratories, USA

11:00 - 11:30 Metabolic Flux in Oil Palm
Umi Salamah Ramli
MPOB, MALAYSIA

11:30 - 12:00 Lignin Biosynthesis: A Candidate for Metabolic Engineering in Woody
Plants
Ron Sederoff
North Carolina State University, USA

12:00 - 12:15 Discussion

12:15 - 13:30 Lunch

13:30 - 16:00 Poster Session II (continued)

16:00 Afternoon Coffee/Refreshments

16:30 - 20:00
SESSION VIII
THERAPEUTIC PROTEINS
Session Chair
Peter Gray, University of New South Wales, AUSTRALIA

16:30- 17:00 Metabolic Engineering in UDP-Galactose and CMP-NANA Pools in CHO
Cells in Order to Improve Glycosylation of Recombinant Glycoproteins
Thomas Ryll
Abgenix, USA

17:00 - 17:30 Optimization of the Synthesis of Recombinant Proteins in Yeast
Juan A. Asenjo
University of Chile. CHILE

17:30 - 18:00 Metabolic Engineering of CHO Cells - Use of Proteomics to Identify
Cellular Changes Associated with Recombinant Protein Expression
Peter Gray
University of New South Wales, AUSTRALIA

18:00 - 18:30 Discussion

SESSION IX
MERCK METABOLIC ENGINEERING AWARD LECTURE
Session Chair
Barry Buckland, Merck Research Labs.

19:00 - 20:00 Merck Award Lecture
Jay Bailey
ETH-Zurich, SWITZERLAND

20:00 - 22:00 Conference Banquet

22:00 - 23:00 Social Hour/Last chance to see Session II Posters

Friday, October 27, 2000

06:30 - 08:30 Buffet Breakfast

08:30 - 12:00 SESSION X
NEW DIRECTIONS IN METABOLIC ENGINEERING
Session Chairs
Matthias Reuss, University of Stuttgart, GERMANY
James Bailey, Swiss Federal Institute of Technology, SWITZERLAND

08:30 - 09:00 Image Informatics in the Quantative Analysis of Cell Physiology
Peter Sorger/Ilya Goldberg
Massachusetts Institute of Technology, USA

09:00 - 09:30 In vitro Metabolic Engineering for Functional Genomics and New Pathway Discovery
Jonathan Dordick
Rensselaer Polytechnic Institute. USA

09:30 - 10:00 Coffee Break

10:00 - 10:30 Metabolic Engineering Analysis of Single Cells
Friedrich Srienc
University of Minnesota, USA

10:30 - 11:00 After a Decade of Progress, an Expanded Role for Metabolic
Engineering
Gregory Stephanopoulos
Massachusetts Institute of Technology, USA

11:00 - 12:00 Conference wrap-up and discussion

12:00 - 13:30 Lunch and Adjournment

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